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Syringes, 1 ml Syringe needles, 25 and 27 gauge Surgical instruments: ne scissors, blunt forceps and sharp forceps Betadine and alcohol swabs Staple sutures and forceps for applying staples Ear-notching forceps or other marking device Nonsterile Athymic mice Styrofoam block Paper towels Protocol (a) Prepare a diagram of the scheme that you will use for implanting the ceramic cubes The rest of the procedure is done at a facility designed for housing and working with athymic rodents Adhere to the rules for the facility, including proper attire, use of laminar ow hoods, use of disinfectant solutions, etc Many of the steps listed here are those used at the Athymic Animal Facility (AAF) at Case Western Reserve University and are included for reference (b) Before entering the interior of the AAF put on shoe covers, mask, cap, and sterile gown and gloves.

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(c) Once inside the clean room, turn the laminar ow hood fan to the high setting (d) Spray entire inside working area of the hood with Clidox disinfectant (e) Leave the spray in hood and get the mouse cages from the room in which they are housed The general rule is to use the oldest available mice rst Use all of the mice in one cage before getting a second cage (if necessary) (f) Wipe down hood, and place the cage inside the hood (g) Spray hands with Clidox, and take a mouse out of the cage (h) Inject mouse with 01 to 015 ml anesthetic per 25 g body weight Injections are made intraperitoneally (IP) with a new 25-gauge needle for each mouse (i) Return mouse to top of cage Inject another mouse if needed.

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If more than 2 mice will be used, inject 1 additional mouse just before you begin the implants on 1 of the previously anesthetized mice (j) Fill out cage card with the cube location and identi cation as indicated on your implant sheet (k) Place a Styrofoam block in the center of the hood and cover it with a sterile towel This will serve as the operating eld Place a second sterile towel adjacent to the block and place the instruments on it (l) Spray hands with Clidox (m) Remove a mouse from the cage and place on the towel over the Styrofoam block Wipe the mouse s back with Betadine and alcohol swabs (n) Inject 01 ml 05% Marcaine (a local anesthetic) subcutaneously with a 27-g needle over the area to be incised with scissors.

E1.1 A number of accounting principles and concepts (such as the matching principle) were discussed in this chapter. For each of the following situations, state which principle or concept is involved. a. A case of food poisoning occurred in a restaurant. The restaurant is being sued by a number of its customers who were hospitalized. The estimated cost that the restaurant is likely to suffer from this lawsuit is disclosed in a footnote because of the __________ principle. b. A hotel has traditionally depreciated its furniture and equipment using the straight-line method. This year a different depreciation method was used without advising its financial statement readers of this change. As a result, it is violating both the __________ principle and the __________ principle. c. A motel s normal payday for employees is every Friday. The yearend occurs on a Monday. The pay earned by employees for those three days is recorded in the motel s accounts because of the __________ principle. d. Last year a remote fishing resort purchased a floatplane to fly guests to the resort. The aircraft cost at that time was $150,000. This year, the plane is worth $160,000. However, it continues to be recorded on the books at $150,000 because of the __________ principle and the __________ principle.

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If adequate general anesthesia has not been obtained, you may inject an additional 005 ml rodent cocktail..

1. Step index (multimode); 2. Graded index (multimode); and 3. Single mode (also called monomode).

#!/usr/bin/perl -w use strict; use XML::Atom::Client; my $username = Ben Hammersley ; my $password = XXXXXXXX ; my $FeedURI = http://www.mediacooperative.com/mtatom.cgi/weblog/ ; my $api = XML::Atom::Client->new; $api->username($username); $api->password($password); my $feed = $api->getFeed($FeedURI); if ($feed) { print $feed->as_xml; } else { print $api->errstr; }

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